Induction of DT-Diaphorase in Riboflavin-Deficient Rats by Xenobiotic Treatment.

Hemopoiesis in riboflavin-deficient rats.

Folate derivatives in the liver of riboflavin--deficient rats.

In a preceding paper of ours,1 it was reported that a marked decrease of N5,10methylenetetrahydrofolate reductase as well as a considerable decrease of N5 methyltetrahydrofolate transferase activity in the liver was found in riboflavin deficient rats. In the present study, chromatographic analysis followed by microbiologic assay of folate derivatives in the liver was made on rats with or withou...

The effects of fenvalerate on hepatic and cerebral xenobiotic metabolizing enzymes in selenium and/or iodine deficient rats

Objective(s): Particularly in developing countries, selenium and/or iodine deficiencies are encountered and use of pesticides in agriculture are not well-controlled. Fenvalerate is a pyrethroid insectide used in agriculture and has applications against a wide range of pests. This study was designed to evaluate the effects of fenvalerate on hepatic and cerebral xenobiotic metabolizing enzyme act...

Properties and reaction mechanism of DT diaphorase from rat liver.

DT diaphorase was purified to homogeneity from rat liver and characterized. The molecular weight of the enzyme was calculated to be 5.0 h 0.06 x lo4 from sedimentation equilibrium experiments and to be 4.8 x IO4 by thin layer gel filtration method using Sephadex G-200. The identity of FAD as a prosthetic group was confirmed by o-amino acid oxidase test. It was found that 1 mole of FAD was prese...

Application of Riboflavin-Deficient Rat Erythrocytes in the in Vitro Assay of Faba Bean Toxicity

Background: Faba bean toxicity (favism) is a common condition observed in our region. Objective: To develop a short and simple technique involving the use of riboflavin-deficient rats to test in vivo faba bean toxicity. Methods/Results: Sprague Dawley rats were maintained on a riboflavin-deficient diet and their vitamin B2 status was monitored by the assay of erythrocyte glutathione reductase (...